The Synergistic Effect of Zuogui Pill and Eldecalcitol on Improving Bone Mass and Osteogenesis in Type 2 Diabetic Osteoporosis

Background and Objectives: The incidence of diabetic osteoporosis, an important complication diabetes mellitus, is increasing gradually. This study investigated the combined effect Zuogui pill (ZGP) eldecalcitol (ED-71), a novel vitamin D analog, on type 2 osteoporosis (T2DOP) explored their action mechanism. Materials Methods: Blood glucose levels were routinely monitored in db/db mice while inducing T2DOP. We used hematoxylin eosin staining, Masson micro-computed tomography, serum biochemical analysis to evaluate changes bone mass blood calcium phosphate mice. Immunohistochemical staining was performed assess osteoblast osteoclast statuses. MC3T3-E1 cell line cultured vitro under high concentration induced undergo osteogenic differentiation. Quantitative real-time polymerase chain reaction, Western blot, immunofluorescence, ALP, alizarin red carried out detect differentiation PI3K–AKT signaling pathway activity. Results: ZGP ED-71 led dramatic decrease increase strongest when both together. with promoted activity inhibited trabecular region. results revealed that synergistically activated pathway. PI3K inhibitor LY294002 or AKT ARQ092 altered synergistic Conclusions: use reduced through pathway, resulting improved mass. Our suggests abovementioned combination constitutes effective treatment for